Measuring Responses of Spinach Leaves in Various Light Waves

Measuring Responses of Spinach Leaves in Various Light Waves Photosynthesis: Measuring the responses of Spinach Leaves in various Light Waves Abstract The object of this study was to measure the amount of Spinach Floating Disks in each of the petri dishes per each experiment set. The experiment was measured by using 100 mL of a 0.2% solution of Sodium Bicarbonate NAHCO3 that was poured into each of the three petri dishes for the two experiments. The 1st experiment tested Carbon Dioxide (CO2) levels created from Sodium Bicarbonate (NAHCO3) in this experiment as to test the hypothesis in comparison to that of Oxygen (O2) to determine as one level increases so will the other. In the 2nd experiment used the working hypothesis which stated that the red dye that simulates the red light spectrum will produce more photosynthesis than that of the blue, whereas green will produce very little if any. Outcomes of the 1st experiment showed that light-dependent photosynthesis reacted to the heat lamp producing more oxygen. Respectively, the red dye had a 100% effective rate of photosynthesis and oxygenation. Introduction According to the Laboratory Investigations for Biology, 2nd Ed. The process of photosynthesis can be summarized in the equation below (John, 2016). Chloroplasts 6CO2 + 12 H2Oà ¯Ã†â€™Ã‚ ¨C6H12O6 + 6H2O + 6 O2 By definition photosynthesis is the process in which some organisms and plants use sunlight to create food from carbon dioxide or CO2 and water or H2O. The products for photosynthesis can be described as the use of carbon dioxide with water to make glucose. A sugar that plants use as a source of food or convert to starch and is stored. A byproduct of this process is oxygen (John, 2016) and (Freeman, et al., 2017). There are two phases in photosynthesis. The 1st phase is known as the light dependent reaction phase. This phase requires a chemical process to occur where the pigment chlorophyll absorbs light energy. It then, in turn use high-energy bonds of ATP and NADPH molecules to complete the process. The 2nd phase of photosynthesis is called carbon-fixation process. This phase does not require light because the energy has already been captured (John, 2016). This phase is similar to the 1st phase in which ATP and NADPH are being used except this process uses carbon dioxide to make glucose. According to (Freeman, et al., 2017) glucose is a simple sugar that is a six-carbon monosaccharide and is the major source for plant food. As stated previously plants can turn glucose a carbohydrate into starch for later use or to reinforce cell walls of the plant. The photosynthesis process as a whole takes place within the chloroplast. Chlorophyll, the green color of plants is a pigment that uses enzymes and other molecules which are required for photosynthesis (John, 2016) Carbon Dioxide (CO2) levels created from Sodium Bicarbonate (NAHCO3) in this experiment is the hypothesis being tested in comparison to that of Oxygen (O2) to determine as one level increases so will the other. If the least amount of diluted NAHCO3 was used up, then the Spinach Leaf will not produce large amounts of Oxygen. This experiment is measuring various strengths of NAHCO3 as the independent variable. Other variables not recorded would include room temperature, the size of the sample, and the health of the plant. In this case the number of the disks floating in the solution is the dependent variable. For this experiment ten punched Spinach Leaf Disks were used for testing purposes. The more oxygen released from each of the disks increases the ability of the disk to float to the surface of the petri dish. This process works because the liquid is being replaced with gaseous compound and regains it buoyancy. For this experiment light was used as the control. According to (Yao, et al., 2017) the increase in photosynthesis rate correlates with the intensity of the light being used. It is also mentioned that a light that is too intense can reduce the photosynthesis rate. Which facilitates an important goal of finding the optimal conditions for cultivation. As explained earlier, the level of development of the chloroplasts is directly affected by photosynthesis. (Yao, et al., 2017) explains that this directly affects the rate of growth of a plant and have different identifying markers under varying light intensities. Leaf morphology which shows plasticity is a reflection of this, where under weak light conditions an experiment on eggplants has shown a specific decrease in of the Datura according to (Mao et al. 2012) taken from (Yao, et al., 2017) in an expanded report on Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. (Farquhar and Sharkey 1982) of the same report by (Yao, et al., 2017) caution high-light intensity can cause serious oxidative damages and destroy the photosynthetic system to leaf tissues. Using the determined information from (Yao, et al., 2017), in this lab the experimenters created a unique experiment not to dissimilar from the Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. To simulate the light wave spectrum of the Red, Blue, and Green (RGB) the experimenters used food coloring to dye the solute. Where the working hypothesis state that the red dye that is used to simulate the red light spectrum will produce more photosynthesis than that of the blue, whereas green will produce very little if any. The dependent variable will again be the number of floating Spinach Leaf Disks. The independent variable in the unique experiment are the various colors being introduced with the heat lamp for light as a form of light spectrum. The control will be the heat lamp. Replication of this experiment will be conducted by the team of experimenters next the unique experiment station in this report. In this experiment if the red manipulated spectrum of light produces the most oxygen then the other independent variables blue and green will produce little to no oxygen. Methods       According the lab manual by (John, 2016); The experimenters were to conduct three separate light tests to determine oxygen production if any which will cause the Spinach Leaf Disks to float. 100 mL of a 0.2% solution of Sodium Bicarbonate NAHCO3 was poured into three petri dishes. The first test measures the amount of floating disks in a light devoid room. In this case the petri dish with ten disks was placed in a drawer as to keep any light from disturbing the experiment. This part of the process is known as the 2nd phase or carbon fixation as mentioned in the introduction of this paper. The second test uses natural room light, or fluorescent tube lights to measure the amount of floating disks. Using a second set of 10 disks in a petri dish the experimenters allowed ample amounts of room light to expose the disks in order to measure the 1st phase in photosynthesis, light-dependency. The last and final test of the three experiments measures the amount of floating disks under a heat lamp. The experimenters waited 30 minutes for each of the experiments before gauging results. Following the lab manual by (John, 2016) for the unique experiment, the testers duplicated the three test process for step three only. Still measuring the amount of floating disks, the experimenters added the RGB dye to each one of petri dishes. Again the 100 mL of a 0.2% solution of Sodium Bicarbonate NAHCO3 was poured into three petri dishes. Each of the three petri dishes were left exposed to heat lamps for 30 minutes. Results The experimenters evaluated each of the initial three tests under three different conditions. Complete darkness, natural light, and under a heat lamp. Phase 1 of photosynthesis consisted of light dependent plants. Plants grown under natural light served as the control. As identified in (Table 1), the control or natural light did not produce any floating disks. While under increased light, the heat lamp the Spinach Leaf Disks thrived and photosynthesized to the extent of 100% of expected results. Phase 2, or the Carbon Fixation process which does not require light had no effect. Carbon Fixation of the plant did not store enough energy to start photosynthesis. In the unique experiment phase 2 was not conducted. The results of the unique experiment that was tested in three different color spectrums; RBG is listed in (Table 2). Predominately the synthesized red spectrum of light produced the most results of the three colors tested. Table 1 Results of Subjecting Spinach Leaf Disks to Different Light Conditions Light # of Disks Floating % of Disks Floating Dark 0 0% Room Light 0 0% Under Lamp 10 100% Table 2. Results of Subjecting Spinach Leaf Disks to Different Color Spectrums Color # of Disks Floating % Disks Floating Red 9 90% Green 3 30% Blue 5 50% Discussion In experiment one carbon dioxide (CO2) levels created from sodium bicarbonate (NAHCO3) in this experiment was used as the hypothesis that was being tested in comparison to that of oxygen (O2) to determine as one level increases so will the other. The hypothesis was accepted in this experiment. As was predicted, the more light that was exposed the more oxygen would be produced which would cause the disks to float. Sources for error in this experiment would include improper set up of the vacuum flasks conducted by the previous lab experimenters. Other errors would include bad sampling techniques of the Spinach Leaf Disk. More time in this experiment would improve or give more accurate results for photosynthesis. It would allow a greater measure of values from the experiment. Also conducting multiple sets of the same experimented in order to gain and average ratio to gauge success or failure. In this experiment the learned outcome was that photosynthesis works in two ways. The 1st is light-dependent photosynthesis and the 2nd was carbon fixation. Secondary outcomes learned in this experiment are that different light spectrums produce different results. Where red would produce more oxygenation that blue or green which produces little to no oxygen. In the Unique Experiment the working hypothesis stated that the red dye that simulates the red light spectrum will produce more photosynthesis than that of the blue, whereas green will produce very little if any. Limitations and error of this experiment will include the same listed above in the 1st experiment as well as how little or how much dye is used in the testing of the light spectrum. With greater time and multiple test of the same experiment averaged values could be derived from the test to give a more accurate result. Cited References John, D. (2016). BSC 2010L Laboratory Manual USF St. Petersburg (1st ed.). Boston MA: Pearson Custom Library. Freeman, S., Quillin, K., Allison, L., Black, M., Podgorski, G., Taylor, E., Carmichael, J. Biological science (6th ed.). Hoboken: Pearson Higher Education, [2017]. YAO, X. (2017). RESEARCH ARTICLE: Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. Journal of Integrative Agriculture, 16(1), 97-105.

Argumentative Paper †Abortion Essay

Abortion is a huge problem throughout the United States. It needs to be stopped. Abortion can be defined as the deliberate termination of a human pregnancy. Abortion is not justified for three reasons. One, having an abortion is taking away an innocent life. Two, there are not many reasons that make it okay to kill an unborn baby. Three, every child needs to be given a chance to live, because every child is wanted. To begin with, having an abortion is taking away an innocent life. It’s not the baby’s fault that it was brought into the world, and it should not be punished for that. Every month, more lives are aborted than the total amount of lives taken in the Vietnam War. Statistics show that 1,500,000 to 2,000,000 babies are aborted annually. The bible tells us that God hates hands that shed innocent blood. God views the unborn as human and precious. He says, in Jeremiah 1:5 of the bible,† Before I formed thee in the belly I knew thee, and before thou camest forth out of the womb I sanctified thee, and I ordained thee a prophet unto the nations.† (Wilson) Next, there are not many reasons that make it okay to abort a child. People may give the following reasons that they think make it ok to have an abortion: rape, deformed children, or that the mother can’t afford a child. In the case of rape, abortion is an act of violence that kills a living human being. The circumstances surrounding the conception do not change this simple reality. In the case of deformed children, there are children of all ages, and adults too, who are alive today, and are living with diseases and disabilities. These physical limitations do not make them any less human. If the mother can’t afford the child, there is a solution. There are many crisis pregnancy care centers that offer financial assistance to pregnant women, and then afterwards they will place them up for adoption if necessary. (â€Å"Poverty, rape, disability†) Finally, every child needs to be given a chance to live his/her life, because every child is a wanted child. Whether the parent wants the child, there are still many people in the world who would love to adopt a child. The National Council for Adoption states that there are â€Å"between one and two million infertile and fertile couples and individuals who would like to adopt children.† There are also a large number of couples whose hearts are ready to adopt terminally ill babies, including babies with AIDS. Adoption is a responsible, helpful solution to abortion. Although the mother still has to carry the baby for the duration of her pregnancy, she is also able to give the baby a chance to live his/her life. No child should be given up because every child is a wanted child. (Gans) In conclusion, adoption is a huge problem in the United States. It is not justified for many reasons. Having an abortion is taking away an innocent life. There are not many reasons that make it okay to have an abortion. Every child in this world is a wanted child. Whether the parent of the child wants the child there is another option other than abortion. Adoption is a great option that saves a child’s life. Works Cited Wilson, Gregg. â€Å"ABORTION AMERICA’S #1 KILLER.† http://www.jesus-is-savior.com/Evils%20in%20America/Abortion%20is%20Murder/abortion_americas_killer.html. Web. 19. Nov.2012. â€Å"Poverty, rape, disability or â€Å"unwantedness† do not morally justify abortion.† http://www.abort73.com/abortion/common_objections/. 2005-2012.Web. 19. Nov. 2012. Gans, Olivia. â€Å"Every child a wanted child and other social arguments.† http://www.nrlc.org/abortion/facts/responseargument3.html.1998. Web.19.Nov.2012.

Patron Saint of Butterflies Essay Essay

The main characters of the story are Honey, and Agnes. The whole book was written in a switch off format which is when Honey and Agnes each write a chapter on what happened in there own thoughts. The story shows a relationship between two girls that slowly grow apart holding tight onto what they have left in their friendship. The characters are Honey, her dad which is Mr. Little, Mrs. Little, Nana Pete, Lillian, Benny, and the owners of Mount Blessing, Mother Veronica and Father Emmanuel. In this story, the characters are in a conflict; Mother Veronica and Father Emmanuel were the ones that were against Lillian, Nana Pete, Honey, and Agnes. There were against each other because Father Emmanuel was punishing the people that lived on Mount Blessing. The story started like this, Agnes was looking for Honey she got into trouble. Honey was punished by Emmanuel because she kissed a boy, on her back; Veronica had carved the word â€Å"harlot†. Soon, Nana Pete (Agnes and Benny’s grandmother) found out about the regulation room where people were being punished at. Nana Pete decides to leave after she found out, when Benny had his finger slammed in the door. Someone had called the police, but because Emmanuel knew he was going to get into trouble if the police come, so he decides to it on himself clumsily. Nana Pete takes him to the hospital where they had to undo everything that Emmanuel had done. Right after Benny had woken up, they escape from Mount blessing where Agnes really doesn’t want to because she wants to become a saint, but Honey dreads to escape that place from the day her was born. Honey had to say goodbye to Winky, the person that Honey owns a utterfly garden with. Honey also left a note about if Agnes ever goes back, Honey made Winky call the cops. They travel across from Connecticut all the way to Texas. On the way, Agnes faced a lot of things that were considered a sin, like eating stuff that are red, watching T. V. and listening to the radio which was all considered sins but Honey did all of them even when she knew it was against the rules on Mount Blessing. Agnes struggles to be with Honey because of their differences, especially when they arrive to the destination and meet a girl amed Lillian. Actually, Lillian is Honey’s mother that had left her when she was only a baby, the story of her mother was complicated. She was wonderful at the violin when she had went to Mount Blessing, so she instantly was accepted into Emmanuel’s inner group which usually take years to do. But at that time she was already pregnant but the people in the inner circle knew nothing about it, when they did, Honey was already born into this world, so they made Lillian go away and let Honey stay with Lillian’s brother, Joseph (Mr.Little). Things turn out pretty well until Nana Pete had died from a heart attacked. Honey and Agnes had a major fight after Agnes was praying for Nana Pete to come back alive, Honey said that it was impossible and had some argument that had caused Honey to hit Agnes. Rushing out the door, Honey hopped into the truck and then drove to the shop Lillian owned. She rushed in causing everyone to suddenly look at her, and then she dragged Lillian back, at that same time Agnes had called her parents to fly here and go pick up Agnes. After her parents arrive, Mr. Little called Lillian the name she was given at Mount Blessing. After Agnes left, that was when Honey found out Lillian was her mother, also she finally knew the reason why Nana Pete treated her like her own grandchild. When Agnes arrived at Mount Blessing, she saw there were rows of police cars parked in front of the regulation room, the cops wanted to see all of the children, alone, that is when Emmanuel said â€Å"remember, we are believers† meaning for them to not tell anything about the room. Because the children were so scared they all didn’t respond to the negative things that were mentioned about Mount Blessing. But then, Agnes thought about what was right and what she should do, slowly with trembling legs, she stood up. At the same time, Honey and Lillian was driving to Mount Blessing, when they arrive, the cops arrived because of the fact that Winky had called the police. A few weeks later, all of the parents were to be kept in order to be questioned, that is when Honey and Agnes stand on the same line, and race each other to the finish line. They raced each other at the end because Agnes wanted to be a saint, and she thought racing was a sin, but she finally loosened up from what Honey had helped her with. I thought this book was the adventurous kind in the beginning, but then did I find out it was about friendship and love. This book was very touching from what Honey had described in one of the chapters about how Agnes had changed from the friend that she use to know to what she is now, a saint to be. I thought that it was outrageous when I read to the part about that they were hit by Emmanuel. To what I think, people should not be punished by what they have done just according to the rules that some guy had made, and I thought that if the kids don’t want to live there, why do their parents make them? In a lot of the chapters, it showed that Agnes had a string that was tied around the waist, and every time she had committed a sin, she would tighten the string, just imagining it I would think would be hard enough for me, but to do it, it takes guts. This booked talked a lot about friendship and trust, and I also thought it was so touching when it told the ending about how they raced each other. It would be hard for me to ever still hold on to the friendship that Agnes and Honey had held on to, I would just have stopped being friends with Agnes instead of trying to convince her back to being normal. I really loved this book because it connected with the experiences that I had, my friend, well more like best friend had started to ‘fit in’ with the crowd, and soon, she pretended that she didn’t ever know me. But before I came to America, I had to say goodbye to her, and she said that the only reason she left me was because I started making new friends and didn’t even talk to her. The misunderstanding was all over, but the things that had already happened could not be solved, so I felt that I was in the same relationship with my best friend just like how Agnes and Honey was. This story could be told to all ages and not to a limited age, it talks about friendship, trust, and most important, holding onto your friend.

Question One. Alcohol Use Disorders (Aud) Is A Health...

Question One Alcohol use disorders (AUD) is a health condition that is diagnosed when alcohol abusers use alcohol to an enormous extent. AUD entails alcohol addiction, and many people who have AUD struggle with alcohol consumption. This disorder is related to alcoholism, or alcohol abuse. The symptoms of AUD include cravings, a strong need to have a drink during the week or weekends, loss of control involving not being able to stop drinking, feeling out of control, shaking, anxiety, withdrawals, and nausea. Alcohol tolerance is the most significant symptom of AUD because an alcoholic will drink an enormous amount of alcohol to feel the effects. Tolerance has a lot to do with the persons drinking history and genetic inheritance (Swift,†¦show more content†¦The alcoholic goes through a withdrawal period that can cause fatal symptoms. Severe symptoms such as hematoma, pneumonia, meningitis, or infection involvement. Sometimes these symptoms are misdiagnosed as medical and not AUD with alco holics (Saitz, 1998). AWS is assessed by the Clinical Institute Withdrawal Assessment for Alcohol SCALE Revised (CIWA-Ar) (Baron, Garbely, Boyd, 2009; Maldonado, 2010) Question Three Biotransformation occurs most often inside the liver Defining â€Å"biotransformation.† The digestive tract is structured, so that chemicals are not absorbed, but pass directly into the circulatory system, but filtered though the liver first. This is called the first pass metabolism (Devane, 2004). Chemicals absorbed from the GI tract while passing though the liver, any toxins in a substance might be isolated and the biotransformation takes place. Biotransformation can only take place if damage to the body has not taken place. The last stage of biotransformation is the elimination stage. Basically, biotransformation changes the substance to water soluble which can be removed from the circulation of the organs by filtering the blood. 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